Reverase (M-MuLV, RNase H minus)

Reverase is a modified enzyme purified from a plasmid in E. coli strain that directs the synthesis of Moloney Murine Leukemia virus (M-MuLV) reverse transcriptase. M-MuLV reverse transcriptase is an RNA or DNA directed DNA polymerase.

Reverase can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single stranded DNA as a template. This enzyme has been genetically altered to remove associated RNase H activity. Removal of the RNase H activity resulted in an increase of full-length cDNA products. Subsequent amplification of cDNA can be completed with Taq DNA Polymerase.