Bacillus subtilis Pgrac01 Expression Vector

Name: Bacillus subtilis Pgrac01 Expression Vector
SKU: PBS001
Price: 1106 USD
Availability: InStock

pHT01 (PBS001)

Sales Price$1,106.00

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Description

The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.

The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.

The B. subtilis Pgrac01 expression system provides an easy-to-handle tool for high yield intracellular protein production with B. subtilis and for secretion of proteins into the culture medium in large amounts. Expression of the gene of interest is regulated by the Pgrac01 promoter fused to the lac operator, allowing the induction by addition of IPTG.

Vectors

pHT01 (PBS001) – IPTG inducible expression vector for intracellular protein production.
pHT43 (PBS002) – secretion vector; derivative of pHT01 with signal sequence of amyQ for protein export.
pHT08 (PBS003) – derivative of pHT01 with C-terminal encoded 8xHis tag.
pHT09 (PBS004) – derivative of pHT01 with C-terminal encoded Strep tag.
pHT10 (PBS005) - derivative of pHT01 with C-terminal encoded c-Myc tag.
pHT1464 (PBS010) – secretion vector; derivative of pHT01 with improved signal sequence of amyQ for extended protein export.

Bacillus subtilis control vectors are also available.

Please note these products require a license for commercial companies. Please contact us for licensing information.

+ Features

Developed for convenient cloning in E. coli and high level expression of heterologous proteins with B. subtilis
Structurally and segregationally stable
Non-pathogenic
No significant bias in codon usage
Can secrete functional extracellular proteins directly into the culture medium
Useful for the construction of metagenomic libraries

+ Applications

Intracellular or secretory protein production with B. subtilis

+ Vectors

Cat#	Vector	Features
PBS001	pHT01	All subsequent vectors are derivatives of pHT01
PBS002	pHT43 (secretion)	amyQ signal sequence for protein export
PBS003	pHT08	C-terminal 8xHis tag
PBS004	pHT09	C-terminal Strep tag
PBS005	pHT10	C-terminal c-Myc tag
PBS010	pHT1464 (secretion)	Improved amyQ signal sequence for extended protein export