HYPE-5 Transfection Kit

HYPE-5™ Transfection Kit is designed to achieve High Yield Protein Expression in mammalian cells.

This kit has been developed for maximum efficiency in HEK293 and CHO cells growing in suspension (flask, spinner and bioreactor) and cultivated in chemically defined medium. It is suitable for other cells as well. These efficient transient expression systems provide a fast, economical alternative to the stable expression systems, which can be costly and time-consuming. Scaling up to larger volumes for production is straightforward with simple and cost-efficient handling steps.

The HYPE-5 kit is composed of HYPE-5 transfection reagent (a Lipofection reagent) and HYPE-Blast reagent, included for improving protein production in CHO cells. 

Figure 1: HYPE-5™ transfection kit achieves higher protein yield than popular commercial transfection reagents in CHO (A) and HEK (B) cells in suspension. Secreted Alkaline Phosphatase (SEAP) was produced by transient transfection using HYPE-5 (HEK293 cells) or HYPE-5 + HYPE-Blast (CHO cells), LP or FHD transfection reagents according to the manufacturer's instructions. Day 3, 5 and 7 supernatants were collected and SEAP activity was measured.