MagSi-DNA Allround Beads, 1.2µm Bead Size

MagSi-DNA Allround Beads are magnetic silica beads with a nanoporous surface optimized for the purification and isolation of nucleic acids.

Due to the superparamagnetic properties and a 1.2µm bead size, the beads sediment slowly collects within 2-3 minutes in a magnetic field. This makes them applicable for both manual and automated/robotic DNA isolation. The magnetic microparticles are intended as a solid phase extraction tool for custom buffer systems based on chaotropic and non-chaotropic binding principles. They can be used for developing your own nucleic acid isolation and extraction methods, such as:

MagSi-DNA Allround Beads reversibly bind DNA and other nucleic acids under sample- and buffer-specific conditions. A solution containing DNA (e.g. lysate) is combined with the beads and an application-specific binding buffer. After incubation, nucleic acids are bound to the silica surface. By applying a suitable magnet to the container (tube/deepwell microplate), the bead pellet is separated from the sample mixture. Unwanted components are further removed by washing steps in a selection of buffers. Finally, nucleic acids are released in a DNase/RNase-free water or buffer solution.

MagSi-DNA Allround Beads are available with a silica surface and with a carboxylated surface. The two surfaces differ in their optimal binding conditions. When developing a new application, it is recommended to try both types in parallel.

Please note: When separation speed is crucial and sufficient homogenization tools are available, 300nm beads may be more suitable due to their short separation time.