RealMOD™ Green W² 2x qPCR Mix

RealMOD Green W² 2x qPCR mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SYBR Green I dye.

This solution contains all the components necessary to perform qPCR: Taq DNA Polymerase, ultrapure dNTPs, MgCl₂ and SYBR Green I dye. The user simply needs to add water, template and primers.

HotStart DNA Polymerase is activated by a 5 minute incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during qPCR setup. The kit has been successfully used to amplify and detect a variety of DNA targets such as genomic DNA, cDNA and plasmid DNA.