TurboLigation Kit

TurboLigation Kit enables quick ligation of blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. 

This kit combines TurboLigase, an ultrapure recombinant T4 DNA Ligase, and 5x TurboLigation buffer, an optimized quick ligation buffer. A 5-minute TurboLigation reaction offers the same efficiency as a traditional 16°C overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment. There is no need to heat-inactivate the TurboLigase, dilute the reaction, or purify the DNA before chemical transformation.