Description
Unique, thermostable polymerase for isothermal amplification and PCR.
SD Polymerase is a novel, artificial polymerase with a strong strand displacement activity and heat stability up to 93°C. With SD Polymerase, the possibilities of isothermal amplification and regular PCR can be combined.
Natural enzymes with a strong strand displacement activity, such as Phi29 or Bst Polymerase, are active below 68°C only. With the high heat stability of SD Polymerase, the efficiency of isothermal amplification can be enhanced by initial denaturation steps to increase the accessibility of single strands. The initial heating-up improves the specificity in applications such as LAMP, library generation, PCDR, WGA and standard PCR.
SD Polymerase is suitable for high GC-content and difficult targets. Long Range PCR can be performed with target sizes up to 30 kb.
This enzyme has 5'-3' polymerase and 5'-3' strand displacement activities, as well as 3'-A-overhangs. It does not have any exonuclease activity.
Multiple formats are available, each of which includes a reaction buffer and MgCl2:
- 10 U/µl
- High concentration, 50 U/µl
- HotStart, 10 U/µl
- Stable up to 93°C; high heat stability improves specificity
- Ideal for high GC-content and difficult targets
- Higher yields, speed and efficiency than Taq Polymerase
- Suitable for amplification of short (100 bp) and long (20 - 30 kb) DNA fragments
- Detection of ≥ 10 DNA molecules
- Can be used with simple and complex templates
- No exonuclease activity
- Isothermal Amplification
- Strand Displacement (LAMP, WGA, RCA, MDA)
- PCDR
- Standard PCR
- Multiplex PCR
- Long Range PCR up to 30 kb
- Real-Time PCR (only with intercalating dyes)
- Library generation
- Genome Sequencing
| Cat# |
Format |
Concentration |
Size |
| 108702 |
Standard |
10 U/µl |
200 units |
| 108710 |
Standard |
10 U/µl |
1,000 units |
| 108800 |
High Concentration |
50 U/µl |
1,000 units |
| 108850 |
High Concentration |
50 U/µl |
5,000 units |
| 108902 |
HotStart |
10 U/µl |
200 units |
| 108910 |
HotStart |
10 U/µl |
1,000 units |
All products are supplied with SD Polymerase, Incomplete Reaction Buffer (10x), and MgCl2 (100 mM).
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