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Back to: E. coli Expression Systems

Broad-Host-Range Vector pBBR RESO (Promoter Trap Vector)

Cat#: RESO01
reso
Sales Price$697.00
Size
5µg
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Description
pBBR RESO is a broad-host-range promoter cloning vector. It was derived from pBBR1MCS, which itself is a modification of the broad-host-range plasmid pBBR1CM. In contrast to other known broad-host-range vectors, pBBR RESO is maintained at a medium copy number and has a reasonable size of about 6.8 kb. It stably replicates in any Gram-negative bacterium studied and is therefore ideal for the isolation and genetic analysis of DNA sequences with promoter activity in the homologous organism. The reporter system used employs the resolvase-mediated excision of a kanamycin (Kan)-resistance gene flanked by two res sequences. Cloning an active promoter results in Kan-sensitive clones.
 
  • Medium copy number and reasonable size
  • Stably replicates in any Gram-negative bacterium
  • Can identify genes under harsh environmental conditions
  • Can detect both constitutively and transiently induced promoters 
  • Identification of promoter sequence of a cloned gene or operon
  • Identification of promoter sequences in random tnpR-gene fusion libraries
  • Identification of genes induced under harsh environmental conditions
  • pBBR RESO vector, lyophilized DNA, 5 µg

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