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Bacillus megaterium Control Vector

Name: Bacillus megaterium Control Vector
SKU: BMEG10C
Price: 146 USD
Availability: InStock

pGFP1522 (BMEG10C)

Sales Price$146.00

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Please note this product requires a license for commercial companies. Please contact us for details.

Description

The Bacillus megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale.

Several vectors are available as positive controls for expression in B. megaterium.

pGFP1522 (BMEG10C) - expresses GFP under the control of xylose inducible PxylA promoter
pRBBm15C (BMEG13C) - encodes protein of levansucrase, HIS-Tag, can be used for secretion
pRBBm13C (BMEG14C) - encodes protein of levansucrase, STREP-Tag, can be used for secretion
pRBBm16C (BMEG15C) - encodes protein of levansucrase, STREPHIS-Tag, can be used for secretion

These vectors have been validated for recombinant production and one-step affinity purification of L. reuteri levansucrase from a growth medium using a B. megaterium expression system.

Please note positive control vectors are available only in combination with a regular B. megaterium expression vector.

To purchase this product, a license is required for commercial companies. Please contact us for more information.

+ Features

Stable, high-yield protein expression
Ideal for both small- and large-scale protein production
Tightly regulated and efficiently inducible xylose operon
No alkaline proteases activity even up to 5 hours after induction
No endotoxins observed

+ Applications

Positive control for B. megaterium expression system

+ Vectors

Cat#	Control Vector	Encoded Protein	Tag
BMEG10C	pGFP1522	GFP	n/a
BMEG13C	pRBBm15C	Lev∆773His	His-Tag
BMEG14C	pRBBm13C	StrepLev∆773	Strep-Tag
BMEG15C	pRBBm16C	StrepLev∆773His	StrepHIS-Tag