Bacillus subtilis Constitutive Expression Vector

The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.
 
The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.

The B. subtilis Constitutive Expression System provides an easy-to-handle tool for high yield protein production with B. subtilis. These B. subtilis/E. coli shuttle vectors were constructed for convenient cloning in E. coli lacIq strains. For subsequent purification of the protein of interest, two vectors are offered encoding for 8xHis- or Strep tag II:

• pHT2133 (PBS008) - pHT2133 vector with C-terminal encoded 8xHis tag
• pHT2134 (PBS009) - pHT2134 vector with C-terminal encoded Strep tag

A control vector is also available. This vector must be purchased in combination with a regular B. subtilis vector:

• pHT2071 (PBS008C) - positive control vector for constitutive intracellular production of B-galactosidase

Please note these products require a license for commercial companies. Please contact us for licensing information.