Bacillus subtilis Food Grade Strain, 1ml

The Bacillus subtilis system was developed as a host for gram-positive bacteria in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.

The Bacillus subtilis system is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. A large body of information concerning transcription, translation, protein folding and secretion mechanisms, genetic manipulation, and large-scale fermentation is available.

The Bacillus Food Grade Expression System was created to make the advantages of the Bacillus expression system also accessible to areas in which antibiotic resistance gene markers are prohibited, such as the food and feed industry. It enables stable vector-based large scale heterologous protein production by an alternative selection, without antibiotics.

The Bacillus Food Grade Selection System is based on the interplay of an endogenous Bacillustoxin EndoA (expressed from vector) and its antitoxin EndoB (expressed from genome). 

Two host strains are available for this system:

• TEA (PBS043) - based on B. subtilis 168 Marburg; recommended for intracellular protein expression and pathway engineering.
• WEA-C (PBS045) - originated from the eightfold extracellular protease-deficient strain B. subtilis WB800N; particularly constructed for secretory protein production. *Plesae note PBS044 replaces PBS044. The only difference is that WEA-C carries a chloramphenicol resistance.

Both strains are suitable hosts for both the food grade low copy expression vector pTTB1 and the high copy expression vector pTTB2.

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