Bacillus subtilis SURE System Strain, 1ml

The SURE® System: Subtilin Regulated gene Expression

Bacillus subtilis is an attractive host for gram-positive bacteria as it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional extracellular proteins directly into the culture medium. It is used in a variety of applications, including agricultural, medical and food biotechnology and the production of recombinant proteins.

Controlled gene expression in B. subtilis is a cost-effective alternative to IPTG induced gene expression. This is achieved with the used of subtilin, a small peptide antibiotic of 32 amino acids produced by B. subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes, which are transcribed from two promoters. Subtilin regulates/activates its own biosynthesis via a two-component regulatory system.

SURE System Strains

• NZ8963 (PBS023) - B. subtilis wild type, subtilin producing strain (ATCC 6633)
• NZ8900 (PBS024) - B. subtilis 168 strain with spaR and spaK integrated into the chromosome at the amyE locus; host strain for subtilin inducible gene expression in B. subtilis
• NZ8901 (PBS025) - B. subtilis 168 with kanamycin gene integrated into the chromosome at the amyE locus; negative control strain
• MC1061 (ELS10610-01) - E. coli strain; intermediate cloning host

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