The ELISA-VIDITEST anti-HHV-6 IgM kit is intended for the serological diagnosis of diseases associated with HHV–6 infection, such as exanthema subitum, acute respiratory illnesses, diarrhea with fever and febrile seizures in infants, heterophile antibody-negative infectious mononucleosis in children, and interstitial pneumonia, encephalitis, meningitis, hepatitis and aplastic anemia in immunodeficient patients. The presence of IgM anti-HHV-6 antibodies indicates ongoing or recent active infection.
The test is intended for HHV-6-specific IgM determination in human sera. It does not differentiate between HHV-6 subtype A and B. The test should be supplemented with the determination of HHV-6-specific IgG antibodies. We recommend the anti-HHV-6 IgG ELISA kit.
The anti-HHV-6 IgM assay is a solid-phase immunoanalytical test. The strips are coated with native HHV-6 antigen. The anti-HHV-6 antibodies, if present in the sera tested, bind to the immobilized antigens. The antibodies in complexes with antigens are later recognized by animal anti-human IgM antibodies labeled with horseradish peroxidase. The attached labeled antibodies are revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react. A mild change in color, if present, may be attributed to the reaction background.
Brief protocol:
- Prepare reagents and samples
- Dispense 100 µl/well standards, control and samples; incubate 60 min. at room temperature; wash 4 times (250 µl/well), aspirate
- Dispense 100 µl/well Px-conjugate; incubate 60 min. at room temperature; wash 4 times (250 µl/well), aspirate
- Dispense 100 µl/well TMB-BF substrate; incubate 20 min. at room temperature
- Dispense 100 µl/well Stop solution
- Read the absorbance at 450 / 620-690 nm within 20 min.
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