Description
The pPICHOLI vectors are designed for heterologous gene expression in the yeast P. pastoris and in the prokaryote E. coli.
The vectors contain an inducible (yeast) alcohol oxidase (AOX) promoter and an E. coli T7 promoter. The vectors also contain sequences allowing autonomous replication both in P. pastoris and E. coli. Due to these components, vector linearization is no longer required and small amounts of DNA are sufficient to successfully transform P. pastoris.
The integrated PARS sequence enables simple recovery of plasmids from yeast. Time-consuming subcloning into several expression vectors, including testing for a successful gene expression, is no longer necessary. A multiple cloning site enables convenient ligation of DNA fragments into the vectors.
pPICHOLI is a dual expression vector that combines eukaryotic and prokaryotic promoter elements. pPICHOLI is provided as three different vectors with a multiple cloning site in three different reading frames to simplify cloning in frames with the tags. 5 vectors are included.
- pPICHOLI-1 (3579 bp) - has two G bases directly upstream of the SalI site
- pPICHOLI-2 (3578 bp) - lacks one of G bases
- pPICHOLI-3 (3577 bp) - lacks both G bases
- pPICHOLI-HA - combines eukaryotic and prokaryotic promoter elements, includes a HA (hemagglutinin) epitope instead of the biotinylation sequence.
- pPICHOLI-C - has no T7 promoter and is not suited for prokaryotic protein expression.
- Efficient, cost-effective, and time-saving protein production in either E. coli or P. pastoris
- Avoids problems such as protein aggregation, denaturation, or accumulation in inclusion bodies
- Gene products toxic to E. coli may be easily expressed in P. pastoris
- Vectors can be used for in vitro transcription/translation of cloned genes
- Powerful eukaryotic expression system shows rapid growth at high densities combined with the strong AOX or CUP1 promoter
- Convenient affinity purification and detection of recombinant proteins
- Purification of recombinant proteins via metal chelate affinity chromatography
- Detection of recombinant proteins in immunoblotting experiments
- Production of functional target proteins
- Synthesis of proteins for crystallization and NMR analysis
- Expression of proteins in high-throughput systems
- pPICHOLI-1 vector DNA, 10 µg
- pPICHOLI-2 vector DNA, 10 µg
- pPICHOLI-3 vector DNA, 10 µg
- pPICHOLI-HA vector DNA, 10 µg
- pPICHOLI-C vector DNA, 10 µg
- AOX5'-Primer, 500 pmol
- AOX3'-Primer, 500 pmol
- CUP5'-Primer, 500 pmol
Search
{{/_source.image}}
{{/_source.showImage}}
