Standard Cloning Vector pBR328

pBR328 is an ampicillin, tetracycline and chloramphenicol resistant, general purpose cloning vector. It is a derivative of pBR325 produced by the deletion of the bom (basis of mobility) site. Due to this deletion, pBR238 is non-mobilizable, making it suitable for situations that require more stringent biological containment. This deletion creates additional unique cloning sites in the chloramphenicol acetyltransferase gene: Pvu II, BspM II and Bal I.