Ni-IDA His-Tag Purification Columns

Name: Ni-IDA His-Tag Purification Columns
SKU: PR-HTK004
Price: 232 USD
Availability: InStock

4 Columns (Cat# PR-HTK004)

Sales Price$232.00

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Description

Mobitec's Ni-IDA columns pre-packed with 1g silica-based resin provide a fast and convenient tool for purification of recombinant polyhistidine-tagged proteins by gravity flow.

The form-stable silica matrix is pre-charged with Ni²⁺ ions and allows purification on the principle of Immobilized Metal Ion Chromatography (IMAC). Binding of proteins is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni²⁺ ions. The chelating group of the Ni-IDA resin is based on IDA (iminodiacetic acid), which enables strong and efficient binding of target protein onto the IMAC matrix.

In contrast to traditional IDA matrices, MoBiTec's Ni-IDA is an optimized matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the absorbent. As a result, MoBiTec's Ni-IDA provides higher target protein purity.

See Also: His6-GFP bacterial expression vector - a positive control vector for reliable testing of His-tagged protein purification from Ni-IDA columns.