pmIrisFP, FLAG®-tagged

pmIrisFP is a cloning and expression vector, encoding for the photoactivatable fluorescent protein mIrisFP.

mIrisFP combines irreversible photoconversion from a green- to a red-emitting form with reversible photo-switching between a fluorescent and a non-fluorescent state in both forms. The protein is an advanced monomeric variant of EosFP that differs from the monomeric mEosFP by four additional mutations: A69V, K145I, F173S and Y189A. 

mIrisFP has excellent properties as a genetically encoded fluorescence marker. It matures with a half-life of 14 min at 37°C, faster than many engineered fluorescent proteins in vitro. In HEK293 cells, mIrisFP green fluorescence appears 15h after transfection, similar to EGFP (13h). mIrisFP fluorescence can be switched on and off reversibly, but also allows for the non-reversible photoconversion from green to red. The dual photoactivation capability of mIrisFP offers enhanced flexibilitiy and enables the combination of pulse-chase experiments with super-resolution imaging.