Xpert Fast HRM (2x MasterMix)

High Resolution Melting (HRM) analysis presents a powerful tool for detecting mutations and polymorphisms in dsDNA samples, including CpG methylation differences. At increasing temperature, in the presence of a fluorescent dye, minute sequence variations between samples result in different DNA strand dissociation behavior. HRM analysis exploits these differences using the melting curve shapes to distinguish between the samples.

 

Xpert Fast HRM (2x Mastermix) contains all of the components except the specific primers and template, for HRM analysis in qPCR. The mix consists of the combination of a highly efficient enzyme with a highly optimized buffer system, which allows for the efficient amplification of GC-rich and AT-rich templates under both normal and fast PCR conditions.  The intercalating dye in the mastermix does not cause PCR inhibition, therefore allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.

 

Different SNPs show differing Tm melt curve shifts. Class I SNPs (C/T and G/A) demonstrate typically large shifts (>.5C), whereas Class IV SNP (A/T) typically have much smaller shifts (<.2C). The high sensitivity and specificity of Xpert Fast HRM allows the user to distinguish all possible SNPs.

 

Storage is -20C for 1 year.