Zymolyase® 20T

The lytic activity of Zymolyase® 20T varies depending on the yeast strain, growth stage of the yeast, or cultural conditions. Under the conditions described below, the lytic activity of Zymolyase® 20T is 20 U/g. 

One unit (U) of lytic activity is defined as the amount that results in a 30% decrease of the absorbance at 800nm of a reaction mixture* after incubation with gentle shaking at 25ºC for 2 hours. A decrease of A₈₀₀ with 60%, equivalent to 2U, corresponds to complete lysis. In other words, 1U of Zymolyase® lyses 3mg of dry weight Saccharomyces cerevisiae in 2 hours under ideal conditions.

*10ml reaction mixture is composed of 1 ml of distilled water, 3 ml of S. cerevisiae (2mg/ml), 5 ml 1/15 M phosphate buffer (pH 7.5), and 1 ml of enzyme solution.