RmesFect mRNA Transfection Reagent

Name: RmesFect mRNA Transfection Reagent
SKU: RM20500
Price: 258 USD
Availability: InStock

500µl (Cat# RM20500)

Sales Price$258.00

Ask a question about this product

Description

RmesFect mRNA Transfection Reagent is specifically designed for in vitro mRNA transfection with high efficiency and low toxicity.

mRNA transfection is advantageous over plasmid DNA delivery in that it does not require nuclear uptake and it is not integrative. It also provides faster protein expression than DNA expression. RmesFect is based on TEE technology (Triggered Endosomal Escape) that combines and exploits the properties of cationic lipids and polymers. The cationic design of RmesFect protects mRNA from degradation for efficient transport directly into the cytosol.

Figure 1: Several cell lines were transfected with 0.5 µg of mRNA encoding GFP protein and RmesFect transfection reagent (ratio 2:1 for RAW264.7,3:1 for COS7 and CHO-K1 and 4:1 for HeLa cells). After 20 min of incubation at room temperature, complexes were added onto the cells in a dropwise manner. 24 H after, transfection efficiency was measured by FACS analysis.

Figure 2: Several cell lines were transfected with mRNA encoding GFP protein and RmesFect transfection reagent. 24 H after, transfection efficiency was measured by fluorescence microscopy.