LPS Extraction Kit

Name: LPS Extraction Kit
SKU: 17141
Price: 210 USD
Availability: InStock

Cat#: 17141

Sales Price$210.00

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Description

Our LPS Extraction Kit is designed to separate and purify Lipopolysaccharides (LPS) present in the outer wall of gram-negative bacteria. This kit can extract LPS from even a small volume of bacterial cells and gives reproducibly high yields of LPS. The protocol takes less than 60 minutes and includes only three steps: lysis of bacterial cells, purification of LPS among the released cell components, and washing to remove salts. This kit provides 100 extractions.

LPS Extraction Yield

LPS was extracted from each G (-) strain of OD600 using the LPS Extraction Kit, and the extracted LPS was quantified using the Purpald Assay. The degree of contamination of the protein was confirmed to be less than 0.2 µg.

Strain	LPS yield (µg)	Protein contamination
S. typhimurium	200 ~ 400	< 0.2 µg
S. enteritidis	90 ~ 250	< 0.2 µg
S. gallinarum	150 ~ 450	< 0.2 µg
E. coli (wild type)	220 ~ 490	< 0.2 µg
E. coli (O:055)	260 ~ 510	< 0.2 µg
E. coli (O:111)	220 ~ 500	< 0.2 µg
E. coli (O:1)	180 ~ 380	< 0.2 µg

+ Features

Isolates and purifies LPS present in gram negative bacteria
Based on phenol water method
Broadly applicable among different gram-negative bacteria
Removes contaminants such as proteins and nucleic acids
Extraction is completed in under 60 minutes

+ Applications

Study of LPS composition and structure
Systematic study of bacteria
Study of immune response of carbohydrate antigen
LPS inhibition drug design study
Antibiotic target study

+ Contents

Lysis buffer, 100ml
Purification buffer, 80ml

+ Literature

LPS Brochure

Boca Scientific is your premiere source for high-quality, innovative solutions for Cell Biology, Molecular Biology, Immunology, genetics and other lab products and reagents. We bring leading-edge products from our own-line and around the world to laboratories in the US and Canada. Our goal is to offer excellent solutions to drive research and discoveries backed by superior customer support.

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