3D-FectIN 3D Transfection Reagent

Name: 3D-FectIN 3D Transfection Reagent
SKU: TN30250
Price: 193 USD
Availability: InStock

250µl (Cat# TN30250)

Sales Price$193.00

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Description

3D-FectIN™ is a 3D Transfection Reagent specifically developed to directly transfect cells cultured in 3D hydrogels. It is suitable for all types of hydrogels and cells.

3D matrices add a third dimension to the cells' environment and can induce significant differences in cellular phenotype and behavior. Scaffold-based 3D matrices activated with 3D-Fect™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. These transfections can be studied in angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, and other areas.

Figure 1: Transfection of various cells on different gels with 3D-fectin.

Figure 2: Collagen-derived hydrogels were loaded with pVectOZ-SEAP complexed with 3D-FectIN™ (1µg / 3 µl) or other transfection reagents according to the manufacturers' recommendations. 3T3 cells were seeded onto the pre-loaded matrix and alkaline phosphatase (SEAP) secretion was analyzed over 4 days.