Description
The Constitutive Gene Expression System for Lactococcus lactis and other lactic acid bacteria, developed at NIZO Food Research, NL, is a food-grade protein expression system. This system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and can be used for bacteria other than Lactococcus lactis. With this system, fermentation, scale-up and downstream processing are simple.
Host Strains
- MG1363 (ELS01363) - L. lactis subsp. cremoris MG1363, plasmid-free progeny of the dairy strain NCDO712. Most widely used host strain for cloning and gene expression in L. lactis.
- NZ3000 (ELS03000) - Standard strain for food grade selection based on the ability to grow on lactose. The lactose operon, that is generally present on plasmids, has been integrated into the chromosome and the lacF gene was deleted. Deletion of the lacF gene makes this strain unable to grow on lactose unless lacF is provided on a plasmid.
- NZ1330 (ELS01330-01) - This host strain is made for food grade expression systems based on an alr selection marker. It was generated by deleting the alr gene, an alanine racemase encoding gene, in the progenitor strain MG1363. Deletion of the alr gene results in auxotrophy for the essential component D-alanine, implying that the strain is unable to grow on media without D-alanine unless alr is provided on a plasmid, and thereby enables the conversion of L-Ala to D-Ala. The alr gene serves as food-grade complementation marker.
Please note these products require a license for commercial companies. Please contact us for licensing information.
- Endotoxin-free, food-grade expression system
- Fewer endogenous and no exogenous proteases
- No spores or inclusion bodies
- Simple fermentation, scale-up and downstream processing
- Suitable for bacteria other than Lactococcus lactis
- Overexpression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products
- Metabolic engineering
- Protein secretion and anchoring in cell envelope
- Large scale applications
| Cat# |
Strain |
Datasheet |
| ELS01363 |
MG1363 |
Pdf |
| ELS03000 |
NZ3000, food grade, ΔlacF |
Pdf |
| ELS01330-01 |
NZ1330, food grade, Δalr |
Pdf |
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Lactococcus lactis Constitutive Expression System Vector
