NICE® Lactococcus lactis Expression Vector

The Nisin controlled gene expression system (NICE®), developed at NIZO Food Research, NL, is a fully food grade system used for controlled gene expression in Lactococcus lactis. The NICE® system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and the tightly controlled gene expression allows the production of toxic proteins to take place. With this system, fermentation, scale-up and downstream processing are simple.

Vectors

• pNZ8148 (ELV00200-01) - broad-host-range vector; Cm^R; nisin A promoter (PnisA) followed by an NcoI site for translational fusions at the ATG. Contains a terminator after the MCS. Sequence adaptation for cloning into NcoI site can result in a change in the second amino acid of a protein.

• pNZ8149 (ELV00300-01) - broad-host-range vector; lacF for food grade selection for growth on lactose; nisA promoter followed by an NcoI site for translational fusions at the ATG. Contains a terminator after the MCS. Sequence adaptation for cloning into NcoI can result in a change in the second amino acid of a protein.

• pNZ8150 (ELV00250-01) - broad-host-range vector; Cm^R; nisA promoter followed by ScaI site for translational fusions precisely at the ATG. Blunt end fragments are generated by PCR and cannot be cut out again after ligation to the ScaI site. Contains a terminator after the MCS.

• pNZ8151 (ELV00310-01) - broad-host-range vector; lacF gene as food grade selection marker; nisA promoter followed by an ScaI site for translational fusions at the ATG start codon. Contains a terminator after the MCS.

• pNZ8152 (ELV00400-01) - broad-host-range vector; alr gene as food grade selection marker, nisA promoter followed by an ScaI site for translational fusion at the ATG. Contains a terminator after the MCS.

• pNZ8008 (ELV00100-01) - reference plasmid for testing the nisin induction in Lactococcus and other lactic acid bacteria genera. A gusA gene without promoter was fused to the nisin A promoter.

• pNZ9530 (ELV00500-01) - low copy plasmid with pAMβ1 origin of replication, which carries the nisR and nisK genes. For cloning in Lactococcus strains and in strains of other lactic acid bacteria genera that do not have the regulatory genes integrated into the chromosome. In this case, for nisin induced expression a dual plasmid system is used: e.g., pNZ9530 (nisRnisK) + pNZ8150 (+insert).

See also: NICE® Lactococcus lactis Secretion Vectors

Please note a license is required for commercial companies. Please contact us for licensing details.