Back to: NICE® L. lactis Expression System

NICE® Lactococcus lactis Secretion Vector

Name: NICE® Lactococcus lactis Secretion Vector
SKU: ELV00600-01
Price: 1464 USD
Availability: InStock

pNZ8120 (ELV00600-01)

Sales Price$1,464.00

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Please note this product requires a license for commercial companies. Please contact us for details.

Description

The Nisin controlled gene expression system (NICE®), developed at NIZO Food Research, NL, is a fully food-grade system used for controlled gene expression in Lactococcus lactis. The NICE® system is advantageous in that it does not contain endotoxins, inclusion bodies, spores or extracellular proteases, and the tightly controlled gene expression allows the production of toxic proteins to take place. With this system, fermentation, scale-up and downstream processing are simple.

Vectors

pNZ8120 (ELV00600-01) - broad-host-range vector; Cm^R; NICE® vector for protein secretion with the signal sequence of the lactococcal cell wall proteinase PrtP; cloning via a NaeI site; mature protein starts with its own first amino acid.

pNZ8121 (ELV00650-01) - broad-host-range vector; Cm^R; NICE® vector for protein secretion with the signal sequence of the lactococcal cell wall proteinase PrtP; cloning via an EcoRV site; mature protein starts with the first amino acid of PrtP (Asp) after the signal cleavage site. This setting can contribute to a greater efficiency in removing the signal peptide.

pNZ8122 (ELV00700-01) - broad-host-range vector; Cm^R; NICE® vector for protein secretion with the signal sequence of the Lactobacillus brevis SlpA protein; cloning via an NruI site; mature protein starts with the first amino acid of SlpA (Ser) after the signal cleavage site. This setting can contribute to a greater efficiency in removing the signal peptide.

pNZ8123 (ELV00750-01) - broad-host-range vector; Cm^R; NICE® vector for protein secretion with the signal sequence of the lactococcal major secreted protein Usp45; cloning via a NaeI site; mature protein starts with its own first amino acid.

pNZ8124 (ELV00800-01) - broad-host-range vector; Cm^R; NICE® vector for protein secretion with the signal sequence of the lactococcal major secreted protein Usp45; cloning via an EcoRV site; mature protein starts with the first amino acid (Asp) of Usp45 after the signal cleavage site. This setting can contribute to a greater efficiency in removing the signal peptide.

Please note a license is required for commercial companies. Please contact us for licensing details.

+ Features

Endotoxin-free, food-grade expression system
Ideal for expression of membrane proteins and secretion of proteins into the medium
Fewer endogenous and no exogenous proteases
No spores or inclusion bodies
Tightly controlled gene expression allows production of toxic proteins
Simple fermentation, scale-up and downstream processing

+ Applications

Production of homologous and heterologous proteins for food, feed, pharmacological and biocatalysis applications
Production of prokaryotic and eukaryotic membrane proteins
Production of exopolysaccharides
Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as, e.g., dehydrogenases and in-situ cofactor regeneration
High-throughput screening for enzyme evolution or enzyme comparison

+ Secretion Vectors

Cat#	Vector	Note
ELV00600-01	pNZ8120	SP PrtP/NaeI
ELV00650-01	pNZ8121	SP PrtP/EcoRV
ELV00700-01	pNZ8122	SP SlpA/NruI
ELV00750-01	pNZ8123	SP Usp45/Nael
ELV00800-01	pNZ8124	SP Usp45/EcoRV