Xpert cDNA Synthesis Supermix

Trace amounts of contaminating genomic DNA (gDNA) in RNA preparations may lead to false positive signals, misjudgment of gene expression levels, and other significant issues. The effective removal of gDNA prior to cDNA synthesis is crucial to ensuring reliable results.

The new Xpert cDNA Synthesis Supermix provides fast, efficient method for the synthesis of high-quality, full-length (up to 8-10kb) first strand cDNA with excellent yields from purified poly(A)+ mRNA or total RNA templates. gDNA eraser is integrated for efficient elimination of any residual DNA (ssDNA, dsDNA, and plasmid DNA) from the RNA sample.

The Supermix is an optimized master mix containing a balanced concentration of oligo(dT) and random hexamer primers, dNTPs, and Xpert Reverse Transcriptase (RNase H-). With all of the  components included in one tube, the Supermix delivers excellent reproducibility, convenience, and reduced risk of handling errors.

The Supermix also contains GRiSP´s RNase inhibitor. This RNase inhibitor has an improved resistance to oxidation compared to human RNase inhibitors. Additionally, it is stable under very low concentrations of DTT, making it an excellent choice for RNA protection.